Biology CRDMO Service

Jiangxi Chiyan Biology Service department has a variety of technical platforms, professional research teams, various advanced biological instruments and biological laboratories. Rochi Pharmaceutical has rich experience in molecular biology, cell biology, in vitro biology, structural biology and other biological fields. Through the enzyme level, cell level determination and other technical platforms, we help customers conduct compound screening, compound characteristics, mechanism of action and biomarker analysis, and help customers accelerate the process of drug research and development.

Structural biology 

The corresponding cooperative Structural Biology laboratory of Jiangxi Chiyan is equipped with a molecular cloning room and a drug discovery and screening platform based on protein crystallography to support structure-based drug development, from the confirmation of new targets to the final confirmation of structures.

Protein expression and purification platform 

The corresponding team of Jiangxi Chiyan has rich experience in recombinant protein expression and purification services, and uses a variety of protein expression systems, including prokaryotic protein expression system, yeast protein expression system, insect cell protein expression system (baculovirus), mammalian cell protein expression system, and has a variety of fusion technologies, which can provide you with a variety of options in protein expression and purification. From scheme design, gene optimization, expression condition optimization to purification technology system to improve the expression level of your target protein.

◆ Insect cell protein expression system

◆ Yeast protein expression system 

◆ E.COLI protein expression system 

◆ CHO cell protein expression system 

◆ Recombinant kinase preparation service

Crystal structure biology based on X-ray 

The determination of the complete three-dimensional structure of protein and its complex assembly is the scientific basis for studying the relationship between molecular structure and function in life activities and revealing the physical and chemical nature of life phenomena. X-ray diffraction of proteins and their complex crystals is one of the main methods to study the three-dimensional fine structure of biological macromolecules.

With the interpenetration of other disciplines, especially driven by hot disciplines such as structural genomics, protein crystallography extends from the analysis of simple three-dimensional structure of proteins to the solution of various biological macromolecules and complex structures, and pays more attention to the study of the interrelationship between structure and function. In pharmaceutical research and development, protein crystallographic structure is widely used in structure-based drug design and has become an important tool for drug design.

Selenomethionine medium

We provided a complete set of M9 selenomethionine (SeMET) medium, which can be used in IPTG-induced Escherichia coli expression system to produce selenomethionine labeled proteins, and protein crystallography studies using multi-wavelength anomalous scattering (MAD) method. This medium simplifies the procedure of E. coli culture, saving a lot of time, manpower and material costs for customers. After the optimization of Jiangxi Chiyan, the structural genome laboratory was able to accelerate protein production; We can also design and optimize the medium according to your needs. The medium does not need to be sterilized and weighed, and can be added one by one according to the attached user manual. M9 high yield medium doubles the production of bacteria, and is more and more widely used in scientific research and biomedical industry.

Cell biology 

Cell level analysis techniques 

● Detection of cytotoxicity 

● Apoptosis detection and analysis 

● Cell migration analysis 

● Cell invasion analysis 

● HTRF cell level detection and analysis 

● Immunostaining analysis 

● Immunofluorescence analysis (96/384 well plate) 

● Reporter gene analysis (green fluorescent protein/luciferase)

● Radioligand receptor binding test 

● Cell uptake analysis based on Crispr-Cas9 stable cell line construction

● RNA interference method 

● MicroRNA overexpression analysis 

● MicroRNA knockout analysis 

● Application analysis of adenovirus/retrovirus/lentivirus vectors 

● PROTAC molecular evaluation in vitro 

● In vitro evaluation of mRNA vaccines 

● Antibody function evaluation in vitro 

● ADC evaluation in vitro 

● T cell transfection and T cell killing analysis 

● NK cell killing, Car-NK analysis

Radioisotope analysis

● Technology platform(³H, ³³P, ³²P, ³⁵S, ¹²⁵I, ¹⁴C)

● Radioactive receptor binding test 

● Radioactive ligand binding test 

● Radioactive uptake test 

● Cell growth test 

● Radioimmunoassay 

● immunoradiometric assay (IRMA)

Computational biology and molecular modeling 

In addition to traditional biological research methods, the corresponding team of Jiangxi Chiyan has also developed more faster and more effective tools, using computers and big data to assist early drug research and development, reducing the workload of drug researchers, shortening the drug development cycle, and reducing the cost of drug development.

Enzymology and molecular interactions 

The corresponding Biology Department of Jiangxi Chiyan has rich experience in the field of in vitro biology, providing a complete set of biological services through enzyme level determination, cell level determination, cell biology, biochemistry, in vitro isotope determination, stable cell line establishment, gene knockout, RNAi and MicroRNA technology.